ABSTRACT Most commercial apple orchards are established on either Marubakaido (Malus prunifolia Borkh.) (high vigor), M-9 (Malus pumila) (low vigor), or a combination of both rootstocks through the intergrafting technique. The Geneva® Series rootstocks were developed as an alternative to orchards modernization. The vigor of rootstocks influences the anatomical xylem formation and, therefore, the canopy cultivar’s hydraulic conductivity. When affected by embolism, hydraulic conductivity harms plant metabolism, reducing crop yield and fruit quality. This study aimed to evaluate four Geneva® Series rootstocks with potential use in southern Brazil, in terms of hydraulic conductivity (K) and percentage loss of conductance (PLC), during the winter period in two different years. The G.213 rootstock presented the best performance for the variables analyzed; however, higher values of xylem functionality loss were observed in G.814.

ABSTRACT Pecan crops has been expanding in recent years, mainly in southern Brazil. Genotypes that compose Brazilian orchards come from the USA and from selections of plants made by Brazilian producers. This study aimed to characterize with microsatellite markers pecan cultivars registered for cultivation in Brazil, including some selections made in the country. It is important to know the genetic variability of the pecan tree, as it facilitates the identification of possible phytotechnical deficiencies due to the genetic similarity between the plants, in addition to helping in the conservation of the species, among other. Thirty-four, out of forty collected accessions, were genotyped with 11 selected SSR (Simple Sequence Repeats) loci. Twenty-four polymorphic alleles were identified. The genetic similarity matrix, based on the Jaccard coefficient, ranged from 0.125 to 1.0; general mean of similarity was 0.46. The cluster analysis, which was carried out by the Unweighted Pair Group Method with Arithmetic Mean (UPGMA), classified pecan accessions into four main groups. Results showed that there is high genetic variability in germplasm evaluated, although some accessions may be duplicates.

ABSTRACT In the last decades, several biochemical and molecular studies in pear (Pyrus spp.) have revealed processes associated with dormancy establishment and release, leading to the identification of several MADS-box family genes related to bud dormancy regulation. Thus, the aim of this study was to evaluate a preliminary expression pattern of the PpDAM13-1 and PpDAM13-2 MADS-box genes in pear leaf buds (Pyrus pyrifolia Nakai, cultivars Hosui and Nijisseiki; and Pyrus communis L., cultivars Rocha and Santa Maria), grown in the subtropical climate of Southern Brazil. Lateral leaf buds were collected from June to September, and RNA was extracted for evaluation of gene expression by reverse transcription polymerase chain reaction (RT-qPCR). It was found that these two genes decreased the expression as leaf buds are release from dormancy. The Hosui cultivar for both genes and the Nijisseiki cultivar for PpDAM13-2 gene showed peaks of expression in early June, indicating that leaf buds are in a transition dormancy phase. The two European cultivars showed this transition dormancy phase between the end-July and the mid-August. It was concluded that the expression analysis of PpDAM13-1 and PpDAM13-2 genes can be used as a marker to evaluate changes of dormancy phase in pear trees in subtropical climate conditions, and it can be suitable to assist management strategies for overcoming pears’ bud dormancy.

ABSTRACT The objective of this study was to investigate the effect of different rates of ethephon on return bloom and yield, and fruit quality of ‘Fuji’ apple trees. The experiment was performed during the 2014/2015 and 2015/2016 growing seasons. Eighteen-year-old Fuji Standard apple trees grafted on M.9 were sprayed with different rates of ethephon (300 mg L-1, 400 mg L-1, and 500 mg L-1) 30 days after full bloom (DAFB) and compared to an unsprayed control. Yield components (number of fruit per tree, yield, fruit weight, and estimated yield) and fruit quality attributes (fruit firmness, total soluble solids and starch-iodine index) were evaluated in both seasons. Return bloom (percentage of flowering spurs and 1-year-shoots) was assessed only the year following the application. Our results show that ethephon increases return bloom and yield of ‘Fuji Standard’ apple trees, without affecting fruit quality attributes.

Resumo O objetivo deste trabalho foi avaliar a influência do uso de radiação UV-C na intensidade de 0,53 KJ m-2, durante o cultivo de morango (Fragaria x ananassa), sobre os parâmetros físico-químicos e fitoquímicos dos frutos. O delineamento experimental utilizado foi inteiramente casualizado, em arranjo unifatorial, com 11, 19, 29 e 39 aplicações de UV-C e sem aplicação de UV-C. A tonalidade dos frutos apresentou menor ângulo ao final do período de estudo. O pH variou em morangos que receberam 39 aplicações de UV-C, e o conteúdo de sólidos solúveis diferiu com 19 aplicações. A atividade antioxidante foi menor nas frutas com 11, 19 e 29 aplicações de UV-C, enquanto o teor de vitamina C foi menor apenas nas frutas que receberam 29 aplicações. O uso de radiação UV-C de 0,53 KJ m-2, durante o cultivo de morango, afeta os parâmetros físico-químicos e fitoquímicos dos frutos, mas não leva à perda de sua qualidade.

Abstract The objective of this work was to evaluate the influence of the use of UV-C radiation at an intensity of 0.53 KJ m-2, during strawberry (Fragaria x ananassa) cultivation, on the physicochemical and phytochemical parameters of the fruits. The used experimental design was completely randomized, in a single-factor arrangement with 11, 19, 29, and 39 UV-C applications and without UV-C application. The hue of the fruits was smaller at the end of the study period. pH varied in strawberries that received 39 applications of UV-C, and the soluble solids content differed with 19 applications. Antioxidant activity was lower in fruits with 11, 19, and 29 UV-C applications, whereas vitamin C content was lower only in fruits that received 29 applications. The use of UV-C radiation at an intensity of 0.53 KJ m-2, during strawberry cultivation, affects the physicochemical and phytochemical parameters of the fruits, but does not cause losses in their quality.

ABSTRACT Pears are the most imported fruit from Brazil and thus, being an important opportunity to Brazilian growers. However, there are still some problems that restrict pear production, such as the lack of suitable rootstocks. The aim of this study was to assess growth, yield efficiency and fruit quality of ‘William’s’ pear grafted on quinces ‘Champion’ and ‘Melliforme’ and P. calleryana. The experiment was performed during 2009/2010 growing season at the Centro Agropecuário da Palma, FAEM/UFPel. The experimental design was a completely randomized block with three replications per treatment. The assessed parameters were trunk cross sectional area, shoot length, yield efficiency, number of fruit per tree, soluble solids, flesh firmness, fruit weight and fruit size. It was found that ‘William’s’ pear is more efficient and less vigorous when grafted on ‘Champion’ quince, thus being a potential scion x rootstock combination for commercial pear planting at medium densities. Moreover, fruit of trees grafted on quince rootstocks accumulated higher amount of soluble solids.

ABSTRACT Thinning is a cultural practice that leads to balance between fruit yield and quality. It is carried out in a short period of time and requires qualified workforce, whose shortage ends up increasing costs. This study aimed at evaluating the thinning effect of metamitron on peach trees at different periods of time after bloom. The experiment was carried out in a commercial orchard of ‘Sensação’ peach trees located in Morro Redondo, Rio Grande do Sul (RS) state, Brazil, in 2015-2016 crops. Treatments were the application of 200 mg L-1 metamitron, 20, 30, 40, 50 and 60 days after full bloom (DAFB), and manual thinning 40 DAFB. Fruit abscission, effective fructification, period of manual and chemical thinning, number of fruits and yield per plant, mean fruit mass and fruit caliber were evaluated. When metamitron was applied 40 DAFB, percentages of fruit abscission and fruit set, besides the number of fruits, were similar to the ones found when manual thinning was carried out. The intensity of the thinning effect of metamitron in peach trees depends on the application period.

A soja é uma das maiores fontes de proteína vegetal da humanidade. É também muito importante na alimentação animal e como matéria-prima industrial. Grandes avanços foram recentemente alcançados em sua transformação genética. O objetivo desta revisão é fornecer uma discussão acerca de importantes fatores que influenciam a transformação de soja mediada por Agrobaterium, incluindo tecidos alvo, condições do tecido vegetal, métodos de injúria, sistemas de regeneração, marcadores de seleção e genes repórter.

Soybean is one of humanity's major sources of plant protein. It is also very important for animal feed and as industrial raw material. Great advances have recently been achieved in its genetic transformation. This review provides a comprehensive discussion of important factors affecting Agrobacterium-mediated soybean transformation including target tissues, plant tissue health, wounding methods, regeneration systems, selectable markers and reporter genes.

The effects of ethylene on plants have been recognized since the Nineteenth Century and it is widely known as the phytohormone responsible for fruit ripening and for its involvement in a number of plant growth and development processes. Elucidating the mechanisms involved in the ripening of climacteric fruit and the role that ethylene plays in this process have been central to fruit production and the improvement of fruit quality. The biochemistry, genetics and physiology of ripening has been extensively studied in economically important fruit crops and a considerable amount of information is available which ranges from the ethylene biosynthesis pathway to the mechanisms of perception, signaling and control of gene expression. However, there is still much to be discovered about these processes and the objective of this review is to present a brief historic account of how ethylene became the focus of fruit ripening research as well as the development and the state-of- art of these studies at both biochemical and genetic levels.